CSF collection and bio banking were required to meet criteria proposed by BioMS EU.20 If these criteria were not referenced, the paper was required to describe CSF sampling, pre analytical handling and storage techniques applied to ensure the samples used were of sufficient quality. Studies also had to use a validated assay, or describe the ELISA technique to satisfy inclusion. Assays with a coefficient of variation >25% were excluded, as were studies where NfL was detectable in less than 85% of either comparator group.

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